Muedzo uyu, wakagadzirwa nevainjiniya vebiomedical uye virologists kubva kuYunivhesiti yeGlasgow, inopa lateral-kuyerera mhedzisiro yakafanana neyeCOVID-19 bvunzo yekumba mukati memaminitsi makumi matatu.
Mune bepa idzva rakaburitswa nhasi mujenari Nature Communications, timu yekutsvagisa inotsanangura maitiro avakaita sisitimu. Inovaka pane zvakamboitika mukukurumidza kuongororwa uye virology paYunivhesiti, ichiunza mhedzisiro ine 98% chokwadi.
Hepatitis C, hutachiona hweropa hunokuvadza chiropa, hunofungidzirwa kubata vanhu vanopfuura mamiriyoni makumi manomwe pasi rose. Utachiona hwehutachiona pachiropa hunononoka, uye varwere vanogona kusaziva kuti vane hutachiona kusvika varwara zvakanyanya nezvinetso zvakaita se cirrhosis kana gomarara.
Kana utachiona hukaonekwa husati hwafambira mberi zvakanyanya, hunogona kurapwa zvakanaka nemishonga isingadhuri, inowanikwa zviri nyore. Zvakadaro, vanosvika makumi masere kubva muzana vevanhu vane hutachiona havazive nezvehutachiona hwavo kusvika matambudziko ekiriniki aitika.
Nekuda kweizvozvo, vanhu vanosvika mazana mana ezviuru pasi rose vanofa nezvirwere zvine chekuita nehepatitis C gore rega rega, vazhinji vacho vangadai vakaponeswa nekutanga kuongororwa uye kurapwa.
Parizvino, utachiona hweHepatitis C hunoonekwa mumarabhoritari pachishandiswa nhanho-nhanho mbiri iyo inoongorora ropa kuvapo kwemasoja ekudzivirira chirwere uye kuona utachiona hweRNA kana maantigens makuru.
Maitiro acho anogona kutora nguva yakawanda yekuunza mhedzisiro, zvichiwedzera mukana wekuti vamwe varwere vanotora bvunzo havadzoke kuti vadzidze nezvemhedzisiro. Kuwana bvunzo kunogumirawo munyika dzine mari yakaderera uye yepakati, uko ruzhinji rwevanhu vane hepatitis C vanogara.
Nepo mimwe miedzo inotakurika inokwanisa kuunza mhedzisiro yakagadziriswa mumakore achangopfuura, huchokwadi hwavo hunogona kushomeka, kunyanya kune akasiyana genotypes evanhu.
Iyo Yunivhesiti yeGlasgow inotungamirwa nechikwata chitsva sisitimu, zvisinei, yakanakira kushandiswa pasirese. Yakagadziridzwa kubva kune yakafanana sisitimu yavakagadzira kuendesa nekukurumidza kuongororwa kwemarariya, iyo yakaedzwa nemhedzisiro inokurudzira muUganda.
Mudziyo uyu unoshandisa mashiti emapepa ewakisi akafanana neakapetwa akapetwa kugadzirira sampuli dzemaitiro anozivikanwa se loop-mediated isothermal amplification, kana LAMP. Maitiro ekupeta mapepa anoita kuti sampuli igadziriswe uye iendeswe kumakamuri madiki matatu mu cartridge, iyo muchina weLAMP unopisa uye unoshandisa kuyedza sampuli kuvapo kwehepatitis C RNA. Nzira iyi iri nyore zvakakwana kuti inokwanisa, mune ramangwana, kuendeswa kumunda, kubva kusampuli yeropa yakatorwa kubva kumurwere kuburikidza nemunwe.
Iyo nzira inotora anenge maminitsi makumi matatu. Mhedzisiro yacho inounzwa kuburikidza neiri nyore kuverenga lateral kuyerera tambo senge nhumbu bvunzo kana yepamba COVID-30 bvunzo, iyo inoratidza mabhendi maviri emhedzisiro yakanaka uye bhendi rimwe reasina kunaka.
Kuti vaedze prototype yavo, boka racho rakashandisa hurongwa kuongorora 100 isingazivikanwe yeplasma yeropa kubva kuvarwere vane chirwere chisingaperi cheHCV uye mamwe masampuli e100 kubva kuHCV-negative varwere, iyo yakaita seboka rinodzora. Iwo masampuli akaedzwa zvakare achishandisa indasitiri-yakajairwa Abbott RealTime hepatitis C assay kusimbisa iyo LAMP mhinduro. Iyo bvunzo dzeLAMP dzakaburitsa mhinduro dzaive 98% chokwadi.
Chikwata ichi chiri kutarisira kushandisa hurongwa uhu mukuyedza kuchamhembe kweAfrica gore rinouya.
Bepa rechikwata, rakanzi 'Loop mediated isothermal amplification sechishandiso chine simba chekukurumidza kuongororwa hutachiona hwehepatitis C', rakabudiswa muNature Communications. Tsvagiridzo iyi yakatsigirwa nemari kubva kuInjiniya nePhysical Sayenzi Research Council (EPSRC), Medical Research Council neWellcome Trust.
ZVOKUBVA MUNYAYA INO:
- The process of paper folding enables the sample to be processed and delivered to three small chambers in a cartridge, which the LAMP machine heats and uses to test the samples for the presence of hepatitis C RNA.
- The technique is sufficiently simple that it has the potential, in future, to be delivered in the field, from a blood sample taken from a patient via fingerprick.
- The results are delivered through an easy-to-read lateral flow strip like a pregnancy test or a home COVID-19 test, which shows two bands for a positive result and one band for a negative.
